a549 cell culture conditioned media  (Exosome Diagnostics)

Journal: Nanomaterials

Article Title: Nanomaterial Exposure, Extracellular Vesicle Biogenesis and Adverse Cellular Outcomes: A Scoping Review

doi: 10.3390/nano12071231

Figure Lengend Snippet: Physicochemical characteristics of NMs, exposure conditions, and biological effects observed in the respective experimental models.

Article Snippet: [ ] , Pt NPs 40–50 nm , A549 Cell-culture-conditioned media , Differential centrifugation and ExoQuick; DLS, NTA, TEM, SEM, EXOCET TM , FP, qRT-PCR, ELISA, BCA , Exosomes 90–100 nm , TSG101, CD81, CD63, CD9 , ↑ Exosome biogenesis ↑ Exosome total protein concentration ↑ Concentrations of TSG101, CD9, CD63, and CD81 proteins, Typical morphology and no significant difference in size were observed.

Techniques: In Vitro, In Vivo, Membrane, Expressing, Concentration Assay, Activity Assay, Migration, Clinical Proteomics, Permeability

Journal: Nanomaterials

Article Title: Nanomaterial Exposure, Extracellular Vesicle Biogenesis and Adverse Cellular Outcomes: A Scoping Review

doi: 10.3390/nano12071231

Figure Lengend Snippet: Overview of the effects of NM exposure on EVs secretion and consequent biological outcomes.

Article Snippet: [ ] , Pt NPs 40–50 nm , A549 Cell-culture-conditioned media , Differential centrifugation and ExoQuick; DLS, NTA, TEM, SEM, EXOCET TM , FP, qRT-PCR, ELISA, BCA , Exosomes 90–100 nm , TSG101, CD81, CD63, CD9 , ↑ Exosome biogenesis ↑ Exosome total protein concentration ↑ Concentrations of TSG101, CD9, CD63, and CD81 proteins, Typical morphology and no significant difference in size were observed.

Techniques: Isolation, Centrifugation, In Vitro, In Vivo, Control, Next-Generation Sequencing, Western Blot, Immunohistochemistry, Expressing, Bradford Protein Assay, Refractive Index, Protein Concentration, Migration, Activation Assay, Filtration, Phospho-proteomics, Enzyme-linked Immunosorbent Assay, Gene Expression, Activity Assay, Spectroscopy